Kinetic modelling of the biochemical 9-octadecenedioic acid production and lipid accumulation using differently functionalised C18:1 substrates

The potential use of different hydrophobic substrates (oleic acid, oleyl alcohol, methyl oleate and rapeseed oil) for the production high concentrations 9-octadecenedioic acid by genetically engineered C. tropicalis ATCC-20962 yeast was researched. For comparison purposes, data growth, intracellular triacylglyceride (TAG) accumulation dicarboxylic (DCA) were obtained mathematical description using both known newly adapted models. Fermentations carried out in an aerated, stirred 2L-fermenter which temperature (30°C) pH (8.2) controlled automatically. Using oleic resulted highest DCA production, i.e. 31.3 g.L−1 144 h, as well TAG concentration (60.5 m%). Mass balance calculations proved that 35 % consumed used accumulation. alcohol oil showed initial rates well, however, after 80 stopped to reach a maximum. In contrast, with substrate, very slow observed without approaching maximum within experimental time.